RNA-seq from gastrocnemius muscles from rats aged 6, 12, 18, 21, 24 and 27 months
RNA-Seq mRNA baseline
Organism:
Rattus norvegicus
Publication
- Solovyeva EM, Ibebunjo C, Utzinger S, Eash JK, Dunbar A et al. (2021) New insights into molecular changes in skeletal muscle aging and disease: Differential alternative splicing and senescence.
Analysis Methods
Pipeline version | iRAP 1.0.3 |
Analyzed Libraries | |
Filtering Step 1 | Discard reads below minimum quality threshold |
Filtering Step 2 | Check of bacterial contamination; discard offending reads |
Filtering Step 3 | Discard reads with common uncalled characters (e.g. N) |
Filtering Step 4 | Remove reads from pair-end libraries that were orphaned by filtering steps 1-3 |
Read Mapping | Against genome reference (Ensembl release: 107) tophat2 version: 2.1.1 |
Gene Quantification | htseq version: 0.11.0 |
Transcript Quantification | kallisto version: 0.42.4 |
Normalized Counts per Gene |
Resources
